Brizel D M, Klitzman B, Cook J M, Edwards J, Rosner G, Dewhirst M W
Dept. of Radiation Oncology, Duke University Comprehensive Cancer Center, Durham, NC 27710.
Int J Radiat Oncol Biol Phys. 1993 Jan 15;25(2):269-76. doi: 10.1016/0360-3016(93)90348-y.
This laboratory has previously used a window chamber model to measure red blood cell velocity in mammary tumors and normal granulation tissues of the F-344 rat. Because red cell flux and hematocrit more accurately reflect the oxygen carrying potential of blood, we used this model to measure these parameters. Red blood cells were labelled with fluorescein isothiocyanate, and 0.2 ml. packed cells were injected intravenously into rats bearing an 8 to 10 day old R-3230 mammary carcinoma. beta-phycoerythrin (0.15 mg.) was also injected and served as a plasma dye to outline the blood vessels. A sample of peripheral blood was then taken and analyzed by flow cytometry to determine the labeled fraction of red blood cells. Flowing tumor and normal tissue vessels were recorded onto a VCR, and these video images were used to determine vascular length and diameter, RBC flux and velocity, and hematocrit. Median vessel diameter and loge (red blood cell flux) were significantly greater in tumors than in normal tissues (p = 0.007 and p < 0.025, respectively). After controlling for these variables, the median tumor hematocrit of 19% was not significantly greater than the median normal tissue hematocrit of 15%. This technique provides a nontoxic and reproducible method that is now being used to assist in the in vivo definition of tumor oxygenation.
本实验室先前使用窗口室模型来测量F-344大鼠乳腺肿瘤和正常肉芽组织中的红细胞速度。由于红细胞流量和血细胞比容能更准确地反映血液的携氧能力，我们使用该模型来测量这些参数。用异硫氰酸荧光素标记红细胞，将0.2 ml压积细胞静脉注射到患有8至10日龄R-3230乳腺癌的大鼠体内。还注射了β-藻红蛋白（0.15 mg），用作血浆染料以勾勒血管轮廓。然后采集外周血样本并通过流式细胞术分析，以确定红细胞的标记比例。将流动的肿瘤和正常组织血管记录到录像机上，这些视频图像用于确定血管长度和直径、红细胞流量和速度以及血细胞比容。肿瘤中的血管中位数直径和自然对数（红细胞流量）显著大于正常组织（分别为p = 0.007和p < 0.025）。在控制这些变量后，肿瘤血细胞比容中位数19%并不显著高于正常组织血细胞比容中位数15%。该技术提供了一种无毒且可重复的方法，目前正用于协助体内肿瘤氧合的定义。
