[通过DEAE-纤维素柱层析法提取的人类大脑皮质蛋白质组分的抗原组成]

[Antigenic composition of protein fractions of the human cerebral cortex extracted by chromatography on DEAE-cellulose].

作者信息

Ignatov S A, Vedernikova L V, Burbaeva G Sh, Lozovskiĭ D V

出版信息

Zh Nevropatol Psikhiatr Im S S Korsakova. 1977;77(2):273-8.

Abstract

In fractionating a phosphate protein extract from the human brain cortex on DEAE-cellulose 10 fractions of the basic acid proteins were received. Analyses of these fractions were conducted by the method of disc electrophoresis in polyacridamide gel with dodecylsulphate Na and a subsequent separate staining of the desitograms of proteins and glycoproteins, precipitation in agar gel, immunoelectrophoresis and autoimmunography with the use of labeled I125 antibodies to rabbit gamma-globulin. In the first 2 basic fractions there were brain specific antigens with electrophoretic mobility of the beta2--gamma globulin. In the first acid fraction--2 antigens: alpha1-globulin and alpha2-globulin of a glycoprotein nature. In subsequent acid fractions--one antigen with a mobility of alpha1-globulin. A study of the antigen activity of these fractions in a complement fixation test on cold with the serum of schizophrenic patients and disseminated sclerosis depicted differences in the activity of the basic and acid fractions.

摘要

在对人脑皮质的磷酸化蛋白质提取物进行分级分离时，在DEAE - 纤维素上获得了10个碱性酸性蛋白质级分。通过在含有十二烷基硫酸钠的聚丙烯酰胺凝胶中进行圆盘电泳，随后对蛋白质和糖蛋白的密度图进行单独染色、琼脂凝胶沉淀、免疫电泳以及使用标记的I125抗兔γ球蛋白抗体进行自身免疫造影等方法，对这些级分进行了分析。在前两个碱性级分中存在具有β2 - γ球蛋白电泳迁移率的脑特异性抗原。在第一个酸性级分中有2种抗原：具有糖蛋白性质的α1 - 球蛋白和α2 - 球蛋白。在随后的酸性级分中有1种具有α1 - 球蛋白迁移率的抗原。在对精神分裂症患者和播散性硬化症患者血清进行的冷补体结合试验中，对这些级分的抗原活性进行研究，结果显示碱性和酸性级分的活性存在差异。