古细菌甲烷八叠球菌属CHTI55的染色体蛋白MC1与DNA结合的化学计量学。

Stoichiometry of the binding of chromosomal protein MC1 from the archaebacterium, Methanosarcina spp. CHTI55, to DNA.

作者信息

Culard F, Laine B, Sautière P, Maurizot J C

机构信息

Centre de biophysique moléculaire, Orléans, France.

出版信息

FEBS Lett. 1993 Jan 11;315(3):335-9. doi: 10.1016/0014-5793(93)81189-7.

DOI:10.1016/0014-5793(93)81189-7

PMID:8422927

Abstract

We have investigated the binding stoichiometry of the chromosomal MC1 protein on DNA using the gel retardation technique. Analysis of the distribution of the complex containing 0, 1, 2, 3 ... bound proteins shows that the protein MC1 interacts with the DNA as a monomer. Binding experiments with short DNA fragments of various lengths shows that the site size is 11 bp in length. These results are compared to those obtained with other chromosomal proteins including HU protein.

摘要

我们使用凝胶阻滞技术研究了染色体MC1蛋白与DNA的结合化学计量。对含有0、1、2、3……个结合蛋白的复合物分布进行分析表明，蛋白MC1作为单体与DNA相互作用。对各种长度的短DNA片段进行的结合实验表明，结合位点大小为11个碱基对。将这些结果与包括HU蛋白在内的其他染色体蛋白的结果进行了比较。