Gardiol D, Gramajo H C, Hirschbein L, de Mendoza D
Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina.
Gene. 1993 Jan 15;123(1):39-44. doi: 10.1016/0378-1119(93)90536-c.
A cassette containing a selectable cat gene and the lacZ gene without its own promoter has been incorporated into the mini-Mu bacteriophage genome. This mini-Mu derivative, referred to as mMu-Bs, can be used in Escherichia coli for the generation of lacZ transcriptional fusions to Bacillus subtilis genes cloned into plasmids. The resultant fusions can be analyzed in B. subtilis either as multicopy plasmids or as a single copy integrated via a Campbell-like recombination into the wild-type locus of the cloned fragment.
一个含有可选择的氯霉素乙酰转移酶基因（cat基因）和不含自身启动子的乳糖操纵子Z基因（lacZ基因）的盒式结构已被整合到微型Mu噬菌体基因组中。这种微型Mu衍生物，称为mMu-Bs，可用于在大肠杆菌中产生与克隆到质粒中的枯草芽孢杆菌基因的lacZ转录融合体。所得的融合体可在枯草芽孢杆菌中作为多拷贝质粒进行分析，也可通过类似坎贝尔重组的方式作为单拷贝整合到克隆片段的野生型位点。
