Engman H W, Kreuzer K N
Department of Microbiology, Duke University Medical Center, Durham, NC.
Gene. 1993 Jan 15;123(1):69-74. doi: 10.1016/0378-1119(93)90541-a.
We deleted the essential gene 24 from the genome of bacteriophage T4. The delta 24 phage is a conditional lethal mutant that can grow only when the host strain supplies the product of gene 24 in trans, or when the phage acquires a functional gene 24 by some type of recombination event. Thus, gene 24 can be used as a selectable marker, for example permitting transposition into the T4 genome and analyses of plasmid-phage recombination [Woodworth and Kreuzer, Mol. Microbiol. 6 (1992) 1289-1296; H.W.E. and K.N.K., manuscript submitted]. We also found that the promoter region of gene 24 allows a low level of autonomous plasmid replication in T4-infected cells, raising the possibility of a previously unrecognized mode of T4 replication initiation.
我们从噬菌体T4的基因组中删除了必需基因24。Δ24噬菌体是一种条件致死突变体，只有当宿主菌株反式提供基因24的产物时，或者当噬菌体通过某种类型的重组事件获得功能性基因24时才能生长。因此，基因24可以用作选择标记，例如允许转座到T4基因组中并分析质粒-噬菌体重组[伍德沃思和克鲁泽，《分子微生物学》6(1992)1289-1296；H.W.E.和K.N.K.，已提交的手稿]。我们还发现，基因24的启动子区域允许在T4感染的细胞中进行低水平的自主质粒复制，这增加了一种以前未被认识的T4复制起始模式的可能性。
