丙酮丁醇梭菌ATCC824丙酮生产途径中编码酶的基因的序列与排列
Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824.
作者信息
Petersen D J, Cary J W, Vanderleyden J, Bennett G N
机构信息
F.A. Janssens Laboratorium voor Genetica, K.V. Leuven, Heverlee, Belgium.
出版信息
Gene. 1993 Jan 15;123(1):93-7. doi: 10.1016/0378-1119(93)90545-e.
The nucleotide sequence of three open reading frames in the acetone-production locus of Clostridium acetobutylicum ATCC824 has been established. The three gene products, corresponding to acetoacetate decarboxylase (EC 4.1.1.4) and both subunits of the acetoacetyl-CoA:acetate/butyrate:CoA transferase (EC 2.8.3.9) are transcribed in two convergently arranged operons. The intervening DNA region separating the two transcripts is characterized by an inverted repeat which appears capable of forming a stem-loop structure functioning as a Rho-independent transcription terminator in both directions.
丙酮丁醇梭菌ATCC824丙酮生产基因座中三个开放阅读框的核苷酸序列已确定。对应于乙酰乙酸脱羧酶（EC 4.1.1.4）以及乙酰乙酰辅酶A：乙酸/丁酸：辅酶A转移酶（EC 2.8.3.9）两个亚基的三种基因产物在两个反向排列的操纵子中进行转录。分隔这两个转录本的中间DNA区域的特征是一个反向重复序列，该序列似乎能够形成一个茎环结构，在两个方向上都作为不依赖Rho的转录终止子发挥作用。
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