Spencer M J, Vestey J P, Tidman M J, McVittie E, Hunter J A
University Department of Dermatology, Royal Infirmary of Edinburgh, Scotland, U.K.
J Invest Dermatol. 1993 Jan;100(1):16-22. doi: 10.1111/1523-1747.ep12349920.
The influence of ultraviolet B irradiation in therapeutic doses on MHC II-positive epidermal cell numbers and their surface MHC II antigen expression was studied quantitatively using light microscopic immunoperoxidase and immunogold electron microscopic techniques. In multiple ultrathin sections through many MHC II-positive epidermal cells from five healthy subjects, prior to ultraviolet exposure, Langerhans cells and indeterminate cells were found to express similar densities of surface MHC II antigens, which were uniformly distributed over the cell surface. The variation in surface MHC II antigen expression on 97 dendritic epidermal cells from one subject was normally distributed. Following a 6-week course of ultraviolet B irradiation, in the same doses as those normally used for the treatment of psoriasis, MHC II-positive epidermal cell numbers were significantly reduced (mean decrease to 51% of the pre-UVB sample; p < 0.001 analysis of variance), but their surface MHC class II antigen density was significantly increased (p < 0.05 analysis of variance). No MHC II-negative Langerhans cells were detected in either the pre- or post-UVB samples.
采用光学显微镜免疫过氧化物酶和免疫金电子显微镜技术，定量研究了治疗剂量的紫外线B照射对MHC II阳性表皮细胞数量及其表面MHC II抗原表达的影响。在对5名健康受试者的多个MHC II阳性表皮细胞进行的超薄切片中，在紫外线照射前，发现朗格汉斯细胞和不定细胞表达相似密度的表面MHC II抗原，这些抗原均匀分布在细胞表面。来自一名受试者的97个树突状表皮细胞表面MHC II抗原表达的变化呈正态分布。在进行为期6周的紫外线B照射疗程后，使用与治疗银屑病通常所用相同的剂量，MHC II阳性表皮细胞数量显著减少（平均减少至紫外线B照射前样本的51%；方差分析p<0.001），但其表面MHC II类抗原密度显著增加（方差分析p<0.05）。在紫外线B照射前或照射后的样本中均未检测到MHC II阴性的朗格汉斯细胞。
