Huberman D, Håkansson C H, Mercke U, Toremalm N G
Acta Physiol Scand. 1977 Jan;99(1):42-7. doi: 10.1111/j.1748-1716.1977.tb10350.x.
A method permitting simultaneous recording in vitro of the intracellular and extracellular ( = mucociliary) activity of respiratory ciliated mucous membrane is presented. Using this method the influence of different temperature levels (20 degrees, 30 degrees, 40 degrees C) upon the tracheal mucous membrane of 15 rabbits has been investigated. It is shown 1) that the extracellular ( = mucociliary) activity increases with rising temperatures whereas the intracellular activity is unaffected and remains constant, indicating a temperature influence upon the respiratory tract mucus rather than a change of the intracellular "pace-maker" mechanism; 2) that the restraining effect of the mucus decreases with rising temperatures but is still 10% of the intracellular frequency at body temperature; 3) that the presented method should be useful for future investigations concerning the effect of air-pollution and pharmacological substances upon respiratory mucous membranes.
本文介绍了一种可在体外同时记录呼吸道纤毛黏膜细胞内和细胞外（即黏液纤毛）活性的方法。利用该方法，研究了不同温度水平（20℃、30℃、40℃）对15只家兔气管黏膜的影响。结果表明：1）细胞外（即黏液纤毛）活性随温度升高而增加，而细胞内活性不受影响且保持恒定，这表明温度对呼吸道黏液有影响，而非细胞内“起搏器”机制发生变化；2）黏液的抑制作用随温度升高而降低，但在体温时仍为细胞内频率的10%；3）所提出的方法应有助于未来有关空气污染和药物物质对呼吸道黏膜影响的研究。
