Kniss D A, Zimmerman P D, Fertel R H, Iams J D
Department of Obstetrics & Gynecology, Ohio State University, College of Medicine, Columbus 43210-1228.
Prostaglandins. 1993 Jan;45(1):27-33. doi: 10.1016/0090-6980(93)90087-n.
Primary cultures of human amnion cells and the amnion-derived cell line WISH were used to evaluate the hypothesis that transforming growth factor-beta (TGF-beta) can modulate epidermal growth factor (EGF)- induced prostaglandin E2 (PGE2) production. Cells were preincubated for 1 hr with TGF-beta (0.0001-10 ng/ml) and then incubated in the presence or absence of EGF (10 ng/ml) for 4 hrs. TGF-beta alone did not stimulate PGE2 synthesis at any dose examined. However, when primary cultures of amnion cells or WISH cells were preincubated with TGF-beta and then challenged with EGF, there was a potentiation of PGE2 production that was much greater than the additive values of TGF-beta or EGF alone. These data suggest that EGF-induced PGE2 production by amnion cells can be modulated by low concentrations of TGF-beta.
人羊膜细胞原代培养物和羊膜来源的细胞系WISH被用于评估转化生长因子-β（TGF-β）能否调节表皮生长因子（EGF）诱导的前列腺素E2（PGE2）产生这一假说。细胞先用TGF-β（0.0001 - 10 ng/ml）预孵育1小时，然后在有或无EGF（10 ng/ml）存在的情况下孵育4小时。在所检测的任何剂量下，单独的TGF-β均未刺激PGE2合成。然而，当羊膜细胞原代培养物或WISH细胞先用TGF-β预孵育，然后用EGF刺激时，PGE2的产生增强，且远大于单独使用TGF-β或EGF时的加和值。这些数据表明，低浓度的TGF-β可调节羊膜细胞中EGF诱导的PGE2产生。
