从蜚蠊（Blaberus discoidalis）血细胞中纯化前酚氧化酶

Durrant H J, Ratcliffe N A, Hipkin C R, Aspan A, Söderhäll K

School of Biological Sciences, University College of Swansea, Singleton Park, Wales, U.K.

Biochem J. 1993 Jan 1;289 ( Pt 1)(Pt 1):87-91. doi: 10.1042/bj2890087.

Pro-phenol oxidase was purified from the haemocytes of the cockroach Blaberus discoidalis by Blue Sepharose chromatography, hydrophobic-interaction chromatography on a Phenyl-Superose column and, finally, gel filtration on a Superose 6 column. Results suggest that the molecule exists as a polymer of identical 76 kDa monomeric units. The enzyme is a glycoprotein with pI of 5.2 and can be converted by trypsin into phenol oxidase.

通过蓝色琼脂糖凝胶色谱法、苯基超琼脂糖柱上的疏水相互作用色谱法以及最后在Superose 6柱上的凝胶过滤，从蟑螂斑蠊的血细胞中纯化出前酚氧化酶。结果表明，该分子以相同的76 kDa单体单元聚合物形式存在。该酶是一种pI为5.2的糖蛋白，可被胰蛋白酶转化为酚氧化酶。