Kataoka H, Urist M R
Department of Surgery and Biological Chemistry, University of California, Los Angeles.
Clin Orthop Relat Res. 1993 Jan(286):262-70.
To promote a high concentration gradient, syngeneic bone marrow and muscle connective tissue cell cultures and subcultures were exposed to bone morphogenetic protein and associated noncollagenous bone matrix proteins (BMP/NCP) in vitro, transferred to diffusion chambers, and then transplanted into the anterior abdominal wall of isogeneic rats. Both muscle- and marrow-derived cells differentiated into cartilage and chondroosteoid on the inside of diffusion chambers. New bone developed on the vascularized outside surfaces in juxtaposition to avascular tissue on the inside. Levels of glycosaminoglycans (GAGs) and DNA synthesis in tissues inside the chambers were sharply elevated. These data, correlated with histologic observations, demonstrate that the BMP/NCP recruits mesenchymal-type cells for a skeletal tissue pathway of development. Muscle-derived cells, as distinguished from marrow-derived cells, originate in a nonosseous environment but differentiate into osteoprogenitor (osteogenic stem) cells and display levels of DNA synthesis almost as high as marrow stroma-derived cells. The rise in DNA synthesis was maximal in the interval from two to three weeks after transplantation. Whether the bone marrow stroma mesenchymal cell target for BMP is only the osteogenic stem cell population or also includes colony-forming units fibroblasts (CFU-F) remains to be investigated by experiments on established cell lines.
为了促进高浓度梯度，将同基因骨髓和肌肉结缔组织细胞培养物及传代培养物在体外暴露于骨形态发生蛋白和相关的非胶原骨基质蛋白（BMP/NCP），转移至扩散室，然后移植到同基因大鼠的前腹壁。肌肉来源和骨髓来源的细胞均在扩散室内侧分化为软骨和软骨样骨。新骨在血管化的外侧表面形成，与内侧的无血管组织相邻。扩散室内组织中糖胺聚糖（GAGs）水平和DNA合成急剧升高。这些数据与组织学观察结果相关，表明BMP/NCP招募间充质型细胞进入骨骼组织发育途径。与骨髓来源的细胞不同，肌肉来源的细胞起源于非骨环境，但分化为骨祖细胞（成骨干细胞），并且显示出几乎与骨髓基质来源细胞一样高的DNA合成水平。DNA合成的增加在移植后两到三周达到最大值。BMP作用的骨髓基质间充质细胞靶点是仅为成骨干细胞群体还是也包括成纤维细胞集落形成单位（CFU-F），仍有待通过对已建立细胞系的实验进行研究。
