人C4b结合蛋白α链的Ser333-Arg356区域参与补体C4b的结合。
The region Ser333-Arg356 of the alpha-chain of human C4b-binding protein is involved in the binding of complement C4b.
作者信息
Hessing M, Kanters D, Takeya H, van 't Veer C, Hackeng T M, Iwanaga S, Bouma B N
机构信息
Department of Haematology, University Hospital Utrecht, The Netherlands.
出版信息
FEBS Lett. 1993 Feb 15;317(3):228-32. doi: 10.1016/0014-5793(93)81281-4.
Human C4b-binding protein (C4BP) functions as a cofactor to factor I in the degradation of C4b and accelerates the decay rate of the C4b2a complex. In this study we describe a monoclonal antibody directed against the alpha-chain of C4BP that inhibits the binding of C4b to C4BP. In order to identify the structural domain of the alpha-chain of C4BP that interacts with C4b, tryptic fragments of C4BP were generated. Amino acid sequence analysis of the fragments revealed that the residues Ser333-Arg356 of the alpha-chain of C4BP contain the epitope of this antibody, and as a consequence, that this part of the alpha-chain of C4BP is likely to be involved in the interaction with C4b.
人C4b结合蛋白（C4BP）在C4b降解过程中作为因子I的辅因子发挥作用，并加速C4b2a复合物的衰变率。在本研究中，我们描述了一种针对C4BPα链的单克隆抗体，该抗体可抑制C4b与C4BP的结合。为了确定C4BPα链中与C4b相互作用的结构域，我们制备了C4BP的胰蛋白酶片段。对这些片段的氨基酸序列分析表明，C4BPα链的Ser333-Arg356残基包含该抗体的表位，因此，C4BPα链的这一部分可能参与了与C4b的相互作用。
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