Matsuki Y, Yamamoto T, Hara K
Department of Periodontology, Niigata University School of Dentistry, Japan.
J Periodontal Res. 1993 Jan;28(1):35-42. doi: 10.1111/j.1600-0765.1993.tb01048.x.
The exact cell type and site(s) involved in interleukin-1 (IL-1) production during gingival inflammation was determined by combining immunohistochemistry and in situ hybridization. IL-1 messenger RNA (mRNA)-expressing cells in human inflamed gingiva were identified as macrophages. The rate of IL-alpha mRNA expression in these macrophages was the same as IL-1 beta mRNA expression. The rate of IL-1 mRNA expression was higher in connective tissue furthest from the pocket epithelium, although more macrophages were present at the connective tissue subjacent to the pocket epithelium. The IL-1 activity in gingival crevicular fluid (GCF) obtained from inflamed gingiva was higher than that from healthy gingiva and decreased after periodontal therapy. The IL-1 activity in GCF was almost completely abolished by the addition of anti-IL-1 alpha antibody but not by anti-IL-1 beta antibody, indicating that IL-1 alpha is the predominant form in GCF. However, the IL-1 activity in GCF was unrelated to the number of IL-1 mRNA-expressing macrophages in the same gingival site where the GCF was obtained at the same time. The results suggest that macrophages in the connective tissue subjacent to the oral epithelium contribute to the production of IL-1 but those in connective tissue subjacent to the pocket epithelium play a different role in the generation of gingival inflammation.
通过结合免疫组织化学和原位杂交技术，确定了牙龈炎症期间白细胞介素-1（IL-1）产生所涉及的具体细胞类型和部位。在人类炎症牙龈中表达IL-1信使核糖核酸（mRNA）的细胞被鉴定为巨噬细胞。这些巨噬细胞中IL-α mRNA的表达率与IL-1β mRNA的表达率相同。尽管在袋上皮下方的结缔组织中有更多巨噬细胞，但在离袋上皮最远的结缔组织中IL-1 mRNA的表达率更高。从炎症牙龈中获取的龈沟液（GCF）中的IL-1活性高于健康牙龈，且在牙周治疗后降低。加入抗IL-1α抗体后，GCF中的IL-1活性几乎完全被消除，而抗IL-1β抗体则无此作用，这表明IL-1α是GCF中的主要形式。然而，GCF中的IL-1活性与在同一时间获取GCF的同一牙龈部位中表达IL-1 mRNA的巨噬细胞数量无关。结果表明，口腔上皮下方结缔组织中的巨噬细胞有助于IL-1的产生，但袋上皮下方结缔组织中的巨噬细胞在牙龈炎症的发生中发挥不同作用。
