Kawamura J, Mazumdar D C, Lubowitz H
Am J Physiol. 1977 Mar;232(3):F286-90. doi: 10.1152/ajprenal.1977.232.3.F286.
Clearance and micropuncture techniques were employed to assess the relationship between renal glucose and sodium reabsorption in the rat. Late proximal tubular fluid was collected from surface nephrons before and at two intervals after the infusion of hyperoncotic albumin by a double recollection technique. Plasma glucose levels were maintained at 28-44 mM throughout. Proximal tubular reabsorptive rates for both glucose and sodium were elevated 20 min after the start of the hyperoncotic infusion. Continued infusion of the hyperoncotic albumin resulted in a natriuresis and a parallel fall in proximal glucose and sodium reabsorption. Changes in maximal glucose reabsorption rates for the whole kidney paralleled changes in glucose reabsorption in surface nephrons. The addition of calcium and magnesium to the hyperoncotic infusate diminished the natriuresis but did not alter the relationship between sodium and glucose reabsorption. These observations indicate a close relationship between proximal tubular glucose reabsorption and sodium reabsorption during hyperoncotic infusion.
采用清除率和微穿刺技术评估大鼠肾脏葡萄糖重吸收与钠重吸收之间的关系。通过双重采集技术，在输注高渗白蛋白之前以及输注后两个时间点，从浅表肾单位收集近端肾小管液。整个过程中血浆葡萄糖水平维持在28 - 44 mM。高渗输注开始20分钟后，近端肾小管对葡萄糖和钠的重吸收率均升高。持续输注高渗白蛋白导致利钠作用，并使近端葡萄糖和钠重吸收平行下降。全肾最大葡萄糖重吸收率的变化与浅表肾单位葡萄糖重吸收的变化平行。在高渗输注液中添加钙和镁可减轻利钠作用，但不改变钠与葡萄糖重吸收之间的关系。这些观察结果表明，在高渗输注期间，近端肾小管葡萄糖重吸收与钠重吸收之间存在密切关系。
