Milan D, Yerle M, Schmitz A, Chaput B, Vaiman M, Frelat G, Gellin J
Laboratoire de Génétique Cellulaire, INRA, Castanet-Tolosan, France.
Cytogenet Cell Genet. 1993;62(2-3):139-41. doi: 10.1159/000133457.
We present here a new PCR-based technique that allows the production of several micrograms of DNA from only 300 flow-sorted chromosomes. During the first two PCR cycles, the annealing temperature is decreased to 30 degrees C, and numerous random loci are amplified under nonspecific conditions. As demonstrated here for pig chromosomes 1 and 18, the PCR products may be used to identify the chromosomal content of the flow-karyotype peaks of any species by fluorescence in situ hybridization.
我们在此展示一种基于聚合酶链式反应（PCR）的新技术，该技术仅从300条流式分选的染色体就能产生数微克的DNA。在最初的两个PCR循环中，退火温度降至30摄氏度，众多随机位点在非特异性条件下被扩增。如此处针对猪的1号和18号染色体所证明的，PCR产物可用于通过荧光原位杂交来鉴定任何物种的流式核型峰的染色体组成。
