Schmitz A, Chaput B, Fouchet P, Guilly M N, Frelat G, Vaiman M
Laboratoire de Cytométrie, DPTE, DSV, Centre d'Etudes Nucléaires, Fontenay aux Roses, France.
Cytometry. 1992;13(7):703-10. doi: 10.1002/cyto.990130706.
Human and swine chromosomes were analyzed separately and as a mix to obtain bivariate flow karyotypes. They were normalized to each other in order to use the human chromosomal DNA content as standard. Our results led to the characterization of the "DNA line" in swine identical to the human "DNA line." Estimation of the DNA content in mega-base pairs of the swine chromosomes is proposed. Chromosomal assignment to the various resolved peaks on the bivariate swine flow karyotype is suggested from the relation between DNA content quantified by flow cytometry and chromosomal size. Swine chromosomes 1, 13, 6, 5, 10, 16, 11, 18, and Y were assigned to peaks A, B, C, K, L, N, O, Q, and Y, respectively. Peaks D and E were assumed to contain chromosomes 2 and 14, but without specific assignment. Similarly, P and M peaks were expected to correspond to chromosomes 12 and 17. Of the remaining chromosomes (3, 7, X, 8, 15, 9, and 4), chromosomes 3, 7, and X, which were assigned previously to peaks F, G, and H, respectively, led us to deduce that chromosomes 15 and 8 belonged to peaks I and J, and chromosomes 9, 4, and X to peak H.
分别对人类和猪的染色体进行分析，并将它们混合分析以获得双变量流式核型。为了以人类染色体DNA含量为标准，对它们进行相互标准化。我们的结果导致了猪中与人类“DNA线”相同的“DNA线”的特征描述。提出了对猪染色体以百万碱基对为单位的DNA含量的估计。根据流式细胞术定量的DNA含量与染色体大小之间的关系，建议将染色体分配到双变量猪流式核型上的各个解析峰。猪的1号、13号、6号、5号、10号、16号、11号、18号和Y染色体分别被分配到峰A、B、C、K、L、N、O、Q和Y。假设峰D和E包含2号和14号染色体，但没有具体分配。同样，预计峰P和M分别对应12号和17号染色体。在其余染色体（3号、7号、X染色体、8号、15号、9号和4号）中，先前分别分配到峰F、G和H的3号、7号和X染色体，使我们推断15号和8号染色体属于峰I和J，9号、4号和X染色体属于峰H。
