绿豆中1-氨基环丙烷-1-羧酸合酶编码多基因家族两个新成员的鉴定
Identification of two new members of the 1-aminocyclopropane-1-carboxylate synthase-encoding multigene family in mung bean.
作者信息
Botella J R, Schlagnhaufer C D, Arteca J M, Arteca R N, Phillips A T
机构信息
Department of Horticulture, Pennsylvania State University, University Park 16802.
出版信息
Gene. 1993 Jan 30;123(2):249-53. doi: 10.1016/0378-1119(93)90132-m.
The key enzyme regulating ethylene biosynthesis in higher plants is 1-aminocyclopropane-1-carboxylate (ACC) synthase. In mung bean (MB), the existence of three genes encoding this enzyme has previously been reported [Botella et al., Plant Mol. Biol. 18 (1992) 793-797], one of which corresponds to a full-length indole-3-acetic acid-inducible cDNA [Botella et al., Plant Mol. Biol. (1992) 425-436]. In this paper we report the cloning of two new genomic sequences coding for ACC synthase in MB (MAC-4 and MAC-5). MAC-4 is 1340 bp long and encodes 388 amino acids (aa) while MAC-5 is 1393 bp long and encodes for 391 aa. Genomic Southern analysis suggests the existence of only one copy of each gene in the genome.
高等植物中调控乙烯生物合成的关键酶是1-氨基环丙烷-1-羧酸（ACC）合酶。此前已有报道称绿豆（MB）中存在三个编码该酶的基因[博特拉等人，《植物分子生物学》18（1992）793 - 797]，其中一个对应全长吲哚-3-乙酸诱导型cDNA[博特拉等人，《植物分子生物学》（1992）425 - 436]。在本文中，我们报道了绿豆中两个编码ACC合酶的新基因组序列（MAC - 4和MAC - 5）的克隆。MAC - 4长1340 bp，编码388个氨基酸（aa），而MAC - 5长1393 bp，编码391个aa。基因组Southern分析表明基因组中每个基因仅存在一个拷贝。
Zotero 插件