Jones ML, Woodson WR
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907-1165, USA.
Plant Physiol. 1999 Feb;119(2):755-64. doi: 10.1104/pp.119.2.755.
We investigated the expression patterns of three 1-aminocyclopropane-1-carboxylate (ACC) synthase genes in carnation (Dianthus caryophyllus cv White Sim) under conditions previously shown to induce ethylene biosynthesis. These included treatment of flowers with 2,4-dichlorophenoxyacetic acid, ethylene, LiCl, cycloheximide, and natural and pollination-induced flower senescence. Accumulation of ACC synthase transcripts in leaves following mechanical wounding and treatment with 2,4-dichlorophenoxyacetic acid or LiCl was also determined by RNA gel-blot analysis. As in other species, the carnation ACC synthase genes were found to be differentially regulated in a tissue-specific manner. DCACS2 and DCACS3 were preferentially expressed in styles, whereas DCACS1 mRNA was most abundant in petals. Cycloheximide did not induce increased accumulation of ACC synthase transcripts in carnation flowers, whereas the expression of ACC synthase was up-regulated by auxin, ethylene, LiCl, pollination, and senescence in a floral-organ-specific manner. Expression of the three ACC synthases identified in carnation did not correspond to elevated ethylene biosynthesis from wounded or auxin-treated leaves, and there are likely additional members of the carnation ACC synthase gene family responsible for ACC synthase expression in vegetative tissues.
我们研究了香石竹（石竹属香石竹品种白狮）中三个1-氨基环丙烷-1-羧酸（ACC）合成酶基因在先前已证明可诱导乙烯生物合成的条件下的表达模式。这些条件包括用2,4-二氯苯氧乙酸、乙烯、LiCl、环己酰亚胺处理花朵，以及自然衰老和授粉诱导的花朵衰老。通过RNA凝胶印迹分析还确定了机械损伤以及用2,4-二氯苯氧乙酸或LiCl处理后叶片中ACC合成酶转录本的积累情况。与其他物种一样，发现香石竹ACC合成酶基因以组织特异性方式受到差异调节。DCACS2和DCACS3在花柱中优先表达，而DCACS1 mRNA在花瓣中最为丰富。环己酰亚胺并未诱导香石竹花朵中ACC合成酶转录本的积累增加，而ACC合成酶的表达在生长素、乙烯、LiCl、授粉和衰老处理下以花器官特异性方式上调。在香石竹中鉴定出的三种ACC合成酶的表达与受伤或生长素处理的叶片中乙烯生物合成的增加并不对应，并且香石竹ACC合成酶基因家族可能还有其他成员负责营养组织中ACC合成酶的表达。
