Robertson J D, Schwartz O M, Lee P
Department of Neurobiology, Duke University School of Medicine Durham, North Carolina 27710.
J Comp Neurol. 1993 Feb 22;328(4):485-500. doi: 10.1002/cne.903280404.
This work aims at a better understanding of the organization of the brain of Octopus vulgaris, emphasizing the touch and visual learning centers. We injected the carbocyanine dye, DiI, into the cerebrobrachial connectives and, separately, into the brachial nerves of living octopuses. In both experiments, retrogradely transported granules of DiI appeared in motor neurons in the superior buccal, posterior buccal and subvertical lobes and in a hitherto unsuspected motor nucleus of several hundred neurons in the posterior dorsal basal and median basal lobes. In addition we labeled afferent fibers by injecting DiI into the caudal (sensory) division of the cerebrobrachial connective on one side; the label spread throughout the superior buccal, posterior buccal and the lateral and median inferior frontal lobes mainly on the injected side. It extended through the cerebral tract into the subvertical lobe, into the superior frontal lobe through the interfrontal tract, through the posterior buccal commissure into the opposite posterior buccal lobe and into the median inferior frontal lobe. The work suggests a new function for the posterior dorsal and median basal lobes, which are shown for the first time to project through the inferior frontal lobe system into the brachial nerves. In addition it represents the first full report of the successful use of the carbocyanine dyes DiI and DiO for labeling nerve tissue in a live invertebrate animal.
这项工作旨在更好地了解普通章鱼大脑的组织结构，重点关注触觉和视觉学习中心。我们将羰花青染料DiI注入活体章鱼的脑臂连接体，并分别注入臂神经。在这两个实验中，逆行运输的DiI颗粒出现在上颊叶、后颊叶和垂直下叶的运动神经元中，以及后背基部叶和中基部叶中一个此前未被怀疑的由数百个神经元组成的运动核中。此外，我们通过将DiI注入一侧脑臂连接体的尾侧（感觉）部分来标记传入纤维；标记主要在注入侧扩散到上颊叶、后颊叶以及外侧和内侧下额叶。它通过脑束延伸到垂直下叶，通过额间束延伸到上额叶，通过后颊连合延伸到对侧后颊叶和内侧下额叶。这项工作揭示了后背叶和中基部叶的新功能，首次表明它们通过下额叶系统投射到臂神经。此外，它是关于羰花青染料DiI和DiO成功用于标记活体无脊椎动物神经组织的第一份完整报告。
