Watanabe J, Asaka Y, Kanamura S
Department of Anatomy, Kansai Medical University, Osaka, Japan.
J Histochem Cytochem. 1993 Mar;41(3):397-400. doi: 10.1177/41.3.8429202.
To study the process of expression of cytochrome P-450 (P-450) in hepatocytes during development, we measured microphotometrically the P-450 content in periportal and perivenular hepatocytes of male rats during peri- and postnatal growth. From Day 19 of gestation to Day 5 after birth, P-450 content in both periportal and perivenular hepatocytes increased markedly (periportal 1046%; perivenular 819%). The content in periportal hepatocytes remained unchanged from 5 to 20 days of age, and increased slightly (24%) from 20 to 45 days of age. However, the content in perivenular hepatocytes increased progressively (105%) between 5 and 45 days of age. The difference in P-450 content became apparent between periportal and perivenular hepatocytes after 7 days of age. The content in periportal or perivenular hepatocytes reached the adult level at 45 days of age. Therefore, the perinatal period is the time at which a marked increase in P-450 occurs in hepatocytes throughout the liver lobule. The subsequent period before weaning is the time at which the sublobular heterogeneous distribution of P-450 appears. The period after weaning is the time at which a slight increase in P-450 content in periportal hepatocytes and a marked increase in the enzyme in perivenular hepatocytes takes place.
为研究细胞色素P - 450（P - 450）在发育过程中肝细胞中的表达过程，我们采用显微光度法测定了雄性大鼠围生期和出生后生长期间门周和中央静脉周围肝细胞中的P - 450含量。从妊娠第19天到出生后第5天，门周和中央静脉周围肝细胞中的P - 450含量均显著增加（门周增加1046%；中央静脉周围增加819%）。门周肝细胞中的含量在5至20日龄时保持不变，在20至45日龄时略有增加（24%）。然而，中央静脉周围肝细胞中的含量在5至45日龄之间逐渐增加（105%）。7日龄后，门周和中央静脉周围肝细胞中P - 450含量的差异变得明显。门周或中央静脉周围肝细胞中的含量在45日龄时达到成年水平。因此，围生期是整个肝小叶肝细胞中P - 450显著增加的时期。断奶前的后续时期是P - 450小叶下不均一分布出现的时期。断奶后的时期是门周肝细胞中P - 450含量略有增加而中央静脉周围肝细胞中该酶显著增加的时期。
