Iwabe T
Department of Obstetrics and Gynecology, Tottori University, Faculty of Medicine, Yonago.
Nihon Sanka Fujinka Gakkai Zasshi. 1993 Feb;45(2):113-8.
Ultrasonography is indispensable for medical treatment, particularly in the field of reproductive medicine. Therefore, it is important to confirm safety when exposing preimplantation embryos to ultrasound. For this purpose, the study was designed to elucidate the bioeffects of pulsed ultrasound on preimplanted mice embryos. One-cell stage embryo, 2-cell stage embryo, morula and expanded blastocysts of mice in vitro cultured were exposed to experimental pulsed ultrasound for 15 minutes in degassed water at 37 degrees C. The ultrasound used in this study was 2MHz; [spatial-average temporal-average intensity (ISATA): 120mW/cm2, maximum intensity (Im): 39.1W/cm2, the peak rarefactional pressure (Pr): 1.0MPa, pulse width: 10 mus' and pulse repetition frequency: 1.0kHz.]. The development to expanded blastocyst and the nucleic acid synthesis in expanded blastocyst were compared in the embryos exposed (study group) and those not exposed (control) to the ultrasound. There were no significant differences between the two groups in the rates of development to expanded blastocysts and the nucleic acid synthesis. The temperature of the irradiated medium in cell container straw did not rise. These results suggest that pulsed ultrasound at the intensity used in this study does not have a harmful effect on early preimplantation embryos.
超声检查在医学治疗中不可或缺，尤其是在生殖医学领域。因此，在将植入前胚胎暴露于超声下时确认其安全性非常重要。为此，本研究旨在阐明脉冲超声对植入前小鼠胚胎的生物效应。将体外培养的小鼠单细胞期胚胎、二细胞期胚胎、桑葚胚和扩张囊胚在37℃的脱气水中暴露于实验性脉冲超声下15分钟。本研究中使用的超声频率为2MHz；[空间平均时间平均强度（ISATA）：120mW/cm²，最大强度（Im）：39.1W/cm²，峰值稀疏压力（Pr）：1.0MPa，脉冲宽度：10μs，脉冲重复频率：1.0kHz。]。比较了暴露于超声的胚胎（研究组）和未暴露于超声的胚胎（对照组）发育至扩张囊胚的情况以及扩张囊胚中的核酸合成情况。两组在发育至扩张囊胚的比率和核酸合成方面没有显著差异。细胞容器吸管中被照射培养基的温度没有升高。这些结果表明，本研究中使用强度的脉冲超声对早期植入前胚胎没有有害影响。
