Fournier B, Ferralli J M, Price P A, Schlaeppi J M
Pharmaceuticals Research Laboratories, CIBA-GEIGY Ltd, Basel, Switzerland.
J Endocrinol. 1993 Jan;136(1):173-80. doi: 10.1677/joe.0.1360173.
The effects of insulin-like growth factor-I (IGF-I) and IGF-II on the human osteoblast cell-line OHS-4 were investigated. Both IGF-I and IGF-II stimulated cell proliferation at nanomolar concentrations and alkaline phosphatase activity was decreased in a dose-dependent manner with either IGF-I or IGF-II. The production of the bone-specific protein osteocalcin was not influenced by either IGF-I or IGF-II. However, they acted synergistically with 1,25-dihydroxy-vitamin D3 at concentrations ranging from 10 to 100 nmol/l. Neither IGF-I nor IGF-II had an effect on either the basal or the parathyroid hormone-stimulated level of adenylate cyclase activity, and likewise they had no effect on phosphodiesterase activity. Binding and cross-linking experiments confirmed the presence of both type-I and type-II IGF receptors on the OHS-4 cells. The present study shows that IGF-I and IGF-II have similar effects on the parameters studied in these osteoblastic cells. They influenced both proliferation and differentiation markers.
研究了胰岛素样生长因子-I（IGF-I）和IGF-II对人成骨细胞系OHS-4的影响。IGF-I和IGF-II在纳摩尔浓度下均刺激细胞增殖，并且IGF-I或IGF-II均可使碱性磷酸酶活性呈剂量依赖性降低。骨特异性蛋白骨钙素的产生不受IGF-I或IGF-II的影响。然而，它们在10至100 nmol/l的浓度范围内与1,25-二羟基维生素D3协同作用。IGF-I和IGF-II对腺苷酸环化酶活性的基础水平或甲状旁腺激素刺激水平均无影响，同样，它们对磷酸二酯酶活性也无影响。结合和交联实验证实OHS-4细胞上存在I型和II型IGF受体。本研究表明，IGF-I和IGF-II对这些成骨细胞中所研究的参数具有相似的作用。它们影响增殖和分化标志物。
