Cribier S, Gulik A, Fellmann P, Vargas R, Devaux P F, Luzzati V
Institut de Biologie Physico-chimique, Paris, France.
J Mol Biol. 1993 Jan 20;229(2):517-25. doi: 10.1006/jmbi.1993.1051.
The lateral diffusion coefficient of fluorescent lipid analogues incorporated in four cubic phases of lipid-water systems was determined by the modulated fringe pattern photobleaching technique. In two of the phases, Q230 and Q224, whose structure is bicontinuous, the diffusion is almost as fast as in the fluid lipid bilayers, and is essentially independent of the chemical nature of the probe. In the other two phases, whose structure consists of disjointed hydrocarbon micelles embedded in a water matrix (phase Q223, type I) and of water-containing micelles embedded in a hydrocarbon matrix (phase Q227, type II), the diffusion coefficient is strongly dependent on the chemical structure of the probe and on the topological type (I or II) of the structure. The conclusion is drawn that in the micellar phases the apparent diffusion mirrors the ability of the probe to hop from micelle to micelle.
采用调制条纹图案光漂白技术测定了掺入脂质 - 水系统四个立方相中的荧光脂质类似物的横向扩散系数。在其中两个结构为双连续的相Q230和Q224中，扩散速度几乎与流体脂质双层中的一样快，并且基本上与探针的化学性质无关。在另外两个相（其结构由嵌入水基质中的不连续烃类胶束组成（Q223相，I型）以及嵌入烃基质中的含水胶束组成（Q227相，II型））中，扩散系数强烈依赖于探针的化学结构以及结构的拓扑类型（I或II）。得出的结论是，在胶束相中，表观扩散反映了探针从一个胶束跳到另一个胶束的能力。
