PHO4-DNA复合物的晶体学表征

Crystallographic characterization of a PHO4-DNA complex.

作者信息

Hakoshima T, Teranishi Y, Ohira T, Suzuki K, Shimizu M, Shirakawa M, Kyogoku Y, Ogawa N, Oshima Y

机构信息

Faculty of Pharmaceutical Sciences, Osaka University, Japan.

出版信息

J Mol Biol. 1993 Jan 20;229(2):566-9. doi: 10.1006/jmbi.1993.1059.

DOI:10.1006/jmbi.1993.1059

PMID:8429567

Abstract

Crystals have been obtained of the DNA-binding domain of the yeast transcription factor PHO4 in complexes with several synthetic fragments of DNA with appropriate cognate sequences. Crystals suitable for X-ray diffraction studies were produced in the case of a complex of the protein with a 17 base-pair fragment of DNA from a solution in polyethylene glycol and calcium chloride. The crystals have the space group of P4(1)2(1)2 or P4(3)2(1)2 with unit cell dimensions a = b = 56.7 A, c = 447.8 A. The diffraction data at 3 A resolution were collected using synchrotron radiation with a Weissenberg camera for macromolecular crystallography.

摘要

已获得酵母转录因子PHO4的DNA结合结构域与几个具有适当同源序列的DNA合成片段形成的复合物的晶体。在该蛋白质与来自聚乙二醇和氯化钙溶液中的17个碱基对DNA片段的复合物的情况下，产生了适合X射线衍射研究的晶体。这些晶体的空间群为P4(1)2(1)2或P4(3)2(1)2，晶胞尺寸a = b = 56.7 Å，c = 447.8 Å。使用用于大分子晶体学的魏森堡相机，利用同步辐射收集了3 Å分辨率的衍射数据。