Dumont C E, Plissonnier D, Guettier C, Michel J B
INSERM U367, Paris, France.
J Surg Res. 1993 Jan;54(1):61-9. doi: 10.1006/jsre.1993.1011.
The effects of glutaraldehyde pretreatment and allograft rejection in arterial grafts were assessed, using iso- and allografts in rats. An in situ glutaraldehyde fixation procedure was used to obtain homogeneous cross-linked vascular biografts. Ten Lewis rats were isografted, ten were isografted with a glutaraldehyde-treated aortic segment, ten were allografted with aortic segments from brown Norway (BN) inbred rats, and ten were allografted with glutaraldehyde-treated BN aortas. The macroscopic and microscopic appearances of the grafts were analyzed 3 weeks after the initial surgery. Immunological injury to the media and the intimal response were quantified morphometrically after monochromatic staining of cell nuclei (hematoxylin after periodic acid), elastin (orcein), and calcification (Von Kossa). Untreated isografts were normal. Untreated allografts showed the classical signs of arterial wall rejection: adventitial inflammatory granuloma, reduced medial thickness and smooth muscle cell density, and greatly increased intimal thickness (P < 0.005). Glutaraldehyde treatment significantly decreased the medial thickness in both iso- and allografts (P < 0.001) and prevented the intimal proliferative response (P < 0.005), but did not change adventitial inflammation. It also induced massive calcification mainly in isografts (P < 0.001). Histomorphological modifications of glutaraldehyde-treated grafts are consistent with a partial protective effect of glutaraldehyde against the rejection process, but also with an induction of a nonspecific inflammatory reaction. Glutaraldehyde-induced cross-linking of the extracellular matrix was responsible for ectopic calcification of the arterial grafts which was independent of the rejection process.
利用大鼠的同基因移植和异基因移植，评估戊二醛预处理和动脉移植物中同种异体移植排斥反应的影响。采用原位戊二醛固定程序获得均匀交联的血管生物移植物。10只Lewis大鼠接受同基因移植，10只接受经戊二醛处理的主动脉段同基因移植，10只接受来自棕色挪威（BN）近交系大鼠的主动脉段异体移植，10只接受经戊二醛处理的BN主动脉异体移植。在初次手术后3周分析移植物的宏观和微观外观。在对细胞核（高碘酸后苏木精）、弹性蛋白（orcein）和钙化（Von Kossa）进行单色染色后，通过形态计量学对中膜的免疫损伤和内膜反应进行定量分析。未处理的同基因移植物正常。未处理的异体移植物表现出动脉壁排斥的典型迹象：外膜炎性肉芽肿、中膜厚度和平滑肌细胞密度降低，以及内膜厚度大幅增加（P<0.005）。戊二醛处理显著降低了同基因移植和异体移植的中膜厚度（P<0.001），并阻止了内膜增殖反应（P<0.005），但未改变外膜炎症。它还主要在同基因移植中诱导大量钙化（P<0.001）。戊二醛处理的移植物的组织形态学改变与戊二醛对排斥过程的部分保护作用一致，但也与非特异性炎症反应的诱导一致。戊二醛诱导的细胞外基质交联是动脉移植物异位钙化的原因，这与排斥过程无关。
