Yamashiro S, Takayama K, Shiku H, Furukawa K
Department of Oncology, Nagasaki University School of Medicine, Japan.
Leuk Res. 1993 Feb;17(2):129-36. doi: 10.1016/0145-2126(93)90057-r.
The protein smg p21A/Krev-1/rap 1A was identified as a ras p21-like small G-protein, having the ability to revert v-Ki-ras transformed NIH 3T3 fibroblasts. The expression level of smg p21A and ras p21s during phorbol ester-induced differentiation of HL-60 and MEG-01 cell lines was analyzed by immuno- and Northern blotting. In both cell lines, levels of smg p21 and ras p21s increased quickly in early phase of differentiation along with the appearance of differentiation phenotypes. They increased 3-4 fold on days 1-2, then decreased gradually. The increasing smg 21 mRNA levels also corresponded with that of products. Among ras mRNAs, Ha-ras and N-ras transcripts increased somewhat faster than smg 21. These small G-proteins may play closely related roles in the differentiation of these leukemia cell lines.
