Frieling T, Cooke H J, Wood J D
Department of Physiology, College of Medicine, Ohio State University, Columbus 43210-1218.
Am J Physiol. 1993 Jan;264(1 Pt 1):G74-80. doi: 10.1152/ajpgi.1993.264.1.G74.
Intracellular microelectrodes were used to investigate the actions of histamine in the submucous plexus of the distal colon of the guinea pig. Three effects resulted from application of histamine to submucous neurons. The first was membrane depolarization associated with increased input resistance and augmented excitability. The second was presynaptic suppression of acetylcholine release at nicotinic synapses. The third occurred during long-term application and consisted of recurrent trains of action potentials associated with periodic depolarization of membrane potential. Pharmacological analysis, with selective agonists and antagonists, suggested mediation of the first and third response by postsynaptic histamine H2 receptors. The second response was mediated by presynaptic histamine H3 receptors. These actions of histamine represent a mechanism for neuroimmune signaling between mucosal mast cells and submucous neurons in gastrointestinal type 1 hypersensitivity reactions to allergens.
细胞内微电极被用于研究组胺对豚鼠远端结肠黏膜下神经丛的作用。将组胺作用于黏膜下神经元会产生三种效应。第一种是膜去极化，伴有输入电阻增加和兴奋性增强。第二种是在烟碱样突触处对乙酰胆碱释放的突触前抑制。第三种效应发生在长期应用过程中，表现为与膜电位周期性去极化相关的反复动作电位序列。使用选择性激动剂和拮抗剂进行的药理学分析表明，第一种和第三种反应由突触后组胺H2受体介导。第二种反应由突触前组胺H3受体介导。组胺的这些作用代表了在胃肠道对过敏原的1型超敏反应中，黏膜肥大细胞与黏膜下神经元之间神经免疫信号传导的一种机制。
