Imai K, Uzu S, Nakashima K, Akiyama S
Branch Hospital Pharmacy, University of Tokyo, Japan.
Biomed Chromatogr. 1993 Jan-Feb;7(1):56-7. doi: 10.1002/bmc.1130070114.
Novel fluorogenic Edman reagents, 7-N,N-dimethylaminosulphonyl-4- (2,1,3-benzoxadiazolyl)isothiocyanate (DBD-NCS) and 7-aminosulphonyl-4-(2,1,3-benzoxadiazolyl)isothiocyanate (ABD-NCS) having no fluorescence themselves were synthesized. The derivatives of amino acids with DBD-NCS fluorescence at 505 nm with excitation at 385 nm. They were separated on a reversed-phase HPLC column and detected at the sub-picomol level. Ala-Phe derivatized with DBD-NCS was cleaved by acid to generate DBD-thiocarbamyl-Ala.
合成了新型荧光埃德曼试剂，即本身无荧光的7-N，N-二甲基氨基磺酰基-4-（2,1,3-苯并恶二唑基）异硫氰酸酯（DBD-NCS）和7-氨基磺酰基-4-（2,1,3-苯并恶二唑基）异硫氰酸酯（ABD-NCS）。氨基酸与DBD-NCS的衍生物在385nm激发下于505nm处发出荧光。它们在反相高效液相色谱柱上分离，并在亚皮摩尔水平进行检测。用DBD-NCS衍生化的丙氨酰-苯丙氨酸经酸裂解生成DBD-硫代氨基甲酰基-丙氨酸。
