多药耐药基因1（MDR1）在原发性胃癌中的表达及其临床意义
MDR1 gene expression and its clinical relevance in primary gastric carcinomas.
作者信息
Wallner J, Depisch D, Gsur A, Götzl M, Haider K, Pirker R
机构信息
Clinic for Internal Medicine I, University of Vienna, Austria.
出版信息
Cancer. 1993 Feb 1;71(3):667-71. doi: 10.1002/1097-0142(19930201)71:3<667::aid-cncr2820710303>3.0.co;2-6.
BACKGROUND
Drug resistance remains a major problem in gastric carcinomas. To evaluate the mechanisms involved in this resistance, the authors determined the expression of the MDR1 gene, a multidrug resistance gene, in primary gastric carcinomas.
METHODS
MDR1 RNA levels of gastric carcinoma specimens (n = 22) were determined by slot blot analysis. An MDR1 cDNA (probe 5A) was used for the hybridization.
RESULTS
MDR1 RNA was detected in 41% of the gastric carcinomas, with high levels in 18% of the specimens. No expression of the MDR3 gene was observed in these tumors. MDR1 gene expression was independent of patient age, tumor localization, and lymph node involvement. However, MDR1 RNA expression was less frequent in locally advanced tumors and was absent in the primary tumors of all six patients who had distant metastases.
CONCLUSIONS
The data indicate that multidrug-resistant cells are present in primary gastric carcinomas and suggest that multidrug resistance might contribute to the clinical drug resistance of these tumors.
背景
耐药性仍是胃癌的一个主要问题。为评估参与这种耐药性的机制，作者测定了原发性胃癌中多药耐药基因MDR1的表达。
方法
通过狭缝印迹分析测定胃癌标本（n = 22）的MDR1 RNA水平。使用MDR1 cDNA（探针5A）进行杂交。
结果
在41%的胃癌中检测到MDR1 RNA，18%的标本中水平较高。在这些肿瘤中未观察到MDR3基因的表达。MDR1基因表达与患者年龄、肿瘤定位和淋巴结受累无关。然而，MDR1 RNA表达在局部晚期肿瘤中较少见，在所有六例有远处转移的患者的原发性肿瘤中均未出现。
结论
数据表明原发性胃癌中存在多药耐药细胞，并提示多药耐药可能导致这些肿瘤的临床耐药。
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