Schilling K, Cumme G A, Hoffmann-Blume E, Hoppe H, Horn A
Friedrich-Schiller-Universität, Medizinische Fakultät, Institut für Biochemie, Jena, Germany.
Clin Chem. 1993 Feb;39(2):251-6.
If buffer/indicator systems are used as optical thermometers in multicuvettes, the temperature resolution is limited by errors of optical measurements produced mainly by variations of pathlength and blank transmittance of the wells. Theoretical and practical approaches show that, in multicuvettes, a between-well temperature resolution of < 0.05 degrees C can be achieved by multiwavelength photometry with use of the Tris/cresol red indicator system. Using up to three absorbances (A0, A1, A2) measured in the same well at different wavelengths for calculation of differences, quotients, and quotients of differences, we found the optimum temperature signal to be In(A1/A2), with equal-ranking absorbances changing with temperature in opposite directions. We have used the method successfully to measure the temperature profiles and temporal dynamics of temperatures at all positions in two types of multicuvettes under controlled conditions, or not controlled thermostatically.
如果缓冲液/指示剂系统用作多比色皿中的光学温度计，温度分辨率会受到光学测量误差的限制，这些误差主要由孔的光程长度变化和空白透光率产生。理论和实践方法表明，在多比色皿中，使用Tris/甲酚红指示剂系统通过多波长光度法可实现小于0.05摄氏度的孔间温度分辨率。利用在同一孔中不同波长下测量的多达三个吸光度（A0、A1、A2）来计算差值、商以及差值的商，我们发现最佳温度信号为In(A1/A2)，具有同等排名的吸光度随温度呈相反方向变化。我们已成功使用该方法在受控条件下或非恒温控制下测量两种类型多比色皿中所有位置的温度分布和温度随时间的动态变化。
