Carroll W E, Jackson R D, Wilcox A A
Res Commun Chem Pathol Pharmacol. 1976 Jun;14(2):387-90.
The present derivation relates, in terms of analytical chemical photometry, the theory of operation of an extremely simple instrument which embodies both the electrical analog solution to Beer's Law of Spectrophotometry and the multiplication of this solution by a constant, such that the voltage reading of the instrument is identical to the concentration of the light absorbing substance being measured. Because the raw output of the instrument is both linear and proportionable, calibration of the instrument is accomplished by appropriately setting and positioning the potential difference between two known standard solutions. The determination of the concentration of an unknown solution is then the direct reading of the voltage it generates in the system. This approach to measurement is called Potentiophotometry, because it is based on potentials generated from light.
就分析化学光度法而言,本推导涉及一种极其简单仪器的工作原理,该仪器既体现了分光光度法中比尔定律的电学模拟解法,又将此解乘以一个常数,使得仪器的电压读数与被测吸光物质的浓度相同。由于仪器的原始输出既是线性的又是成比例的,因此通过适当设置和定位两种已知标准溶液之间的电位差来完成仪器的校准。然后,未知溶液浓度的测定就是直接读取它在系统中产生的电压。这种测量方法称为电位光度法,因为它基于光产生的电位。