Shapira O M, Mor E, Reshef T, Pfeffermann R A, Cohen I R
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
J Clin Invest. 1993 Feb;91(2):388-90. doi: 10.1172/JCI116211.
Administration of attenuated, activated autoimmune T lymphocytes to syngeneic mice and rats has been shown to prevent or induce remission of experimental autoimmune diseases specific for the autoimmune T cells. The process has been termed "T cell vaccination." In a recent study, T cell vaccination was done using T cells sensitized to rat alloantigens. The procedure produced a significant reduction of the mixed lymphocyte reaction (MLR) against allogeneic cells. The reduction in MLR was not specific: Vaccination with T cells specific for stimulator cells of one allotype led to a reduced MLR stimulated by cells of another allotype. The present study was undertaken to examine whether T cell vaccination can induce tolerance to transplantation antigens in vivo. We used the model of heterotopic cardiac transplantation in rats. We now report that vaccinating rats with syngeneic, activated, alloantigen-primed T lymphocytes significantly prolonged survival of rat cardiac allografts. The effect of T cell vaccination was most evident when the T cells had been obtained from rats specifically sensitized against the donor rats: Brown-Norway (BN) allografts in control Wistar rats survived 8.5 +/- 0.4 d while BN allografts survived 29.2 +/- 7.1 d in Wistar rats that had been vaccinated with Wistar anti-BN cells. Vaccination of Wistar rats with Wistar anti-hooded T cells prolonged survival of BN heart allografts to a lesser but significant degree (13.0 +/- 1.1 d). Thus, T cell vaccination of recipients can prolong survival of allografts.
已证明，将减毒、活化的自身免疫性T淋巴细胞接种于同基因小鼠和大鼠，可预防或诱导针对自身免疫性T细胞的实验性自身免疫疾病缓解。该过程被称为“T细胞疫苗接种”。在最近一项研究中，使用对大鼠同种异体抗原致敏的T细胞进行T细胞疫苗接种。该程序使针对同种异体细胞的混合淋巴细胞反应（MLR）显著降低。MLR的降低并非特异性的：用针对一种同种异型刺激细胞的特异性T细胞进行疫苗接种，会导致另一种同种异型细胞刺激的MLR降低。本研究旨在探讨T细胞疫苗接种是否能在体内诱导对移植抗原的耐受性。我们使用大鼠异位心脏移植模型。我们现在报告，用同基因、活化、经同种异体抗原致敏的T淋巴细胞对大鼠进行疫苗接种，可显著延长大鼠心脏异体移植的存活时间。当T细胞取自对供体大鼠特异性致敏的大鼠时，T细胞疫苗接种的效果最为明显：对照Wistar大鼠中的Brown-Norway（BN）异体移植存活8.5±0.4天，而在接种了Wistar抗BN细胞的Wistar大鼠中，BN异体移植存活29.2±7.1天。用Wistar抗带帽T细胞对Wistar大鼠进行疫苗接种，可使BN心脏异体移植的存活时间延长至较小但显著的程度（13.0±1.1天）。因此，对受体进行T细胞疫苗接种可延长异体移植的存活时间。
