Bancroft A J, Grencis R K, Else K J, Devaney E
Liverpool School of Tropical Medicine, UK.
J Immunol. 1993 Feb 15;150(4):1395-402.
BALB/c mice immunized with radiation attenuated third stage larvae of the filarial nematode Brugia pahangi are strongly immune to challenge infection. Investigation of the profile of cytokines secreted by spleen cells from immune mice stimulated in vitro with either parasite Ag or with Con A revealed high levels of IL-5 and IL-9 and moderate levels of IL-4. In contrast, secretion of IFN-gamma by spleen cells from immune animals was negligible. Spleen cells from control mice secreted low levels of all cytokines assayed. Levels of parasite-specific IgE were significantly elevated in immune animals and a peripheral blood eosinophilia was observed, which exhibited a biphasic distribution. Our results are consistent with the preferential expansion of Th2 cells in immune animals and provide the basis for dissecting the means by which radiation attenuated larvae of filarial nematodes stimulate immunity.
用辐射减毒的丝虫线虫彭亨布鲁线虫第三期幼虫免疫的BALB/c小鼠对攻击感染具有很强的免疫力。对用寄生虫抗原或刀豆球蛋白A体外刺激的免疫小鼠脾细胞分泌的细胞因子谱进行研究发现，白细胞介素-5（IL-5）和白细胞介素-9（IL-9）水平较高，白细胞介素-4（IL-4）水平中等。相比之下，免疫动物脾细胞分泌的γ干扰素（IFN-γ）可忽略不计。对照小鼠的脾细胞分泌的所有检测细胞因子水平都很低。免疫动物体内寄生虫特异性免疫球蛋白E（IgE）水平显著升高，并且观察到外周血嗜酸性粒细胞增多，呈现双相分布。我们的结果与免疫动物中辅助性T细胞2（Th2）的优先扩增一致，并为剖析丝虫线虫辐射减毒幼虫刺激免疫的方式提供了基础。
