Simon F, Rahimy C, Krivine A, Levine M, Pepin J M, Lapierre D, Denamur E, Vernoux L, De Crepy A, Blot P
Laboratoire de Virologie, Hôpital Bichat-Cl. Bernard, Paris, France.
J Acquir Immune Defic Syndr (1988). 1993 Feb;6(2):201-7.
Differences in avidity between HIV-1 antibodies transmitted passively and antibodies synthesized by children born to HIV-1-positive mothers can be measured using a commercially available competitive enzyme immunoassay kit. The avidity determination method is based on the competition between an anti-HIV-1-peroxidase-labeled antibody at a stable and known concentration and the anti-HIV-1 antibodies (IgA, IgG, IgM) present in the child's serum at various and increasing dilutions. The shift in the competition/dilution curves between serum samples taken at the third and the sixth month of the child's life showed either the loss or the synthesis of anti-HIV-1 antibodies. The antibody avidity determination combined with a test detecting free or complexed p24 antigen is a workable and inexpensive serological method for the follow-up of children born to seropositive mothers. Combining these two complementary methods, HIV-1 infection has been established at 6 months of age in 13 of 13 infants, and positive results were confirmed by coculture and by PCR. An HIV-1 infection was excluded at 6 months of age in 17 of 17 infants, results otherwise confirmed by virological and clinical follow-up. These new and convenient approaches to the diagnosis of vertically acquired HIV-1 could be used worldwide, including in developing countries.
可使用市售的竞争性酶免疫分析试剂盒来测定被动传播的HIV-1抗体与HIV-1阳性母亲所生儿童合成的抗体之间的亲和力差异。亲和力测定方法基于稳定且已知浓度的抗HIV-1过氧化物酶标记抗体与儿童血清中不同稀释度且不断增加的抗HIV-1抗体（IgA、IgG、IgM）之间的竞争。在儿童出生后第三个月和第六个月采集的血清样本竞争/稀释曲线的变化显示了抗HIV-1抗体的丧失或合成。抗体亲和力测定与检测游离或复合p24抗原的试验相结合，是一种可行且廉价的血清学方法，用于对血清阳性母亲所生儿童进行随访。结合这两种互补方法，13名婴儿中有13名在6个月大时被确诊为HIV-1感染，通过共培养和PCR证实了阳性结果。17名婴儿中有17名在6个月大时排除了HIV-1感染，病毒学和临床随访进一步证实了结果。这些用于诊断垂直感染HIV-1的新的便捷方法可在全球范围内使用，包括在发展中国家。
