Kuhlmann J, Geisler C
Department of Clinical Immunology, Tissue Typing Laboratory, Copenhagen, Denmark.
Scand J Immunol. 1993 Feb;37(2):271-5. doi: 10.1111/j.1365-3083.1993.tb01766.x.
The multichain T-cell receptor is composed of at least six different polypeptide chains. The clonotypic Ti heterodimer (Ti alpha beta or Ti gamma delta) is non-covalently associated with the CD3 chains (CD3 gamma delta epsilon zeta). The exact number of subunits constituting the T-cell receptor is still not known. It has been suggested that each T-cell receptor contains two Ti dimers. To gain insight into the structure of the T-cell receptor we constructed a Ti alpha beta, Ti gamma delta double positive T-cell line which contained four functional Ti chains (Ti alpha, beta, gamma, and delta). The data demonstrated an absence of Ti dimers containing mixtures of chains other than the typical Ti alpha beta and Ti gamma delta combinations. Furthermore, by co-modulation experiments we demonstrated that the Ti alpha beta and the Ti gamma delta dimers were not expressed in the same T-cell receptor. Our data indicate that the T-cell receptor does not contain two Ti dimers.
多链T细胞受体由至少六种不同的多肽链组成。克隆型Ti异二聚体（Tiαβ或Tiγδ）与CD3链（CD3γδεζ）非共价结合。构成T细胞受体的亚基的确切数量仍不清楚。有人提出每个T细胞受体包含两个Ti二聚体。为了深入了解T细胞受体的结构，我们构建了一个Tiαβ、Tiγδ双阳性T细胞系，其中包含四条功能性Ti链（Tiα、β、γ和δ）。数据表明不存在包含典型Tiαβ和Tiγδ组合以外的链混合物的Ti二聚体。此外，通过共调节实验，我们证明Tiαβ和Tiγδ二聚体不在同一T细胞受体中表达。我们的数据表明T细胞受体不包含两个Ti二聚体。
