Kong R K, Barrios A, Knapp W, Macher B A
Department of Chemistry and Biochemistry, San Francisco State University, California 94132.
Arch Biochem Biophys. 1993 Feb 1;300(2):677-83. doi: 10.1006/abbi.1993.1094.
Our efforts to determine the carbohydrate binding specificity of two myeloid-specific monoclonal antibodies (VIM-1 and VIM-10) resulted in the purification of three fucosylated glycosphingolipids from human chronic myelogenous leukemia cells. After repeated high-performance liquid chromatographic separations, two forms of fucosylated glycosphingolipids were resolved. VIM-1 and VIM-10 were found to bind to the heptaosylceramide Gal beta 1-4 (Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer with the Le(x) (Lewis X) epitope, but not to either the hexaosylceramide Fuc alpha 1-2Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1-Cer or the octaosylceramide Fuc alpha 1-2Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer, each with the Le(y) (Lewis Y) epitope. The latter two glycosphingolipids are the first Le(y) antigens to be purified from human leukocytes and structurally characterized. Binding studies with a range of glycosphingolipids from myeloid cells and other biological sources demonstrated that VIM-1 and VIM-10 bind to Le(x) glycosphingolipids with five or more sugar residues, but not to a glycosphingolipid (III3Fuc-nLc6Cer) with an internal Le(x) trisaccharide.
我们致力于确定两种髓系特异性单克隆抗体（VIM-1和VIM-10）的碳水化合物结合特异性，从人慢性髓性白血病细胞中纯化出了三种岩藻糖基化糖鞘脂。经过反复的高效液相色谱分离，分离出了两种形式的岩藻糖基化糖鞘脂。发现VIM-1和VIM-10与具有Le(x)（刘易斯X）表位的庚糖神经酰胺Galβ1-4（Fucα1-3）GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer结合，但不与具有Le(y)（刘易斯Y）表位的己糖神经酰胺Fucα1-2Galβ1-4（Fucα1-3）GlcNAcβ1-3Galβ1-4Glcβ1-1-Cer或辛糖神经酰胺Fucα1-2Galβ1-4（Fucα1-3）GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer结合。后两种糖鞘脂是首次从人白细胞中纯化并进行结构表征的Le(y)抗原。对一系列来自髓系细胞和其他生物来源的糖鞘脂进行的结合研究表明，VIM-1和VIM-10与具有五个或更多糖残基的Le(x)糖鞘脂结合，但不与具有内部Le(x)三糖的糖鞘脂（III3Fuc-nLc6Cer）结合。
