Miura S, Hasumi K, Endo A
Department of Applied Biological Science, Tokyo Noko University, Japan.
FEBS Lett. 1993 Feb 22;318(1):88-90. doi: 10.1016/0014-5793(93)81334-v.
The antibiotic patulin was found to inhibit protein prenylation in mouse FM3A cells. Thus, the agent reduced incorporation of [3H]mevalonate into proteins by 50% at a concentration of 7 microM. In a cell-free assay, patulin inhibited rat brain farnesyl:protein transferase, one of the enzymes responsible for protein prenylation. The inhibition was 50% at a concentration of 290 microM.
已发现抗生素展青霉素可抑制小鼠FM3A细胞中的蛋白质异戊二烯化。因此，该药物在浓度为7微摩尔时可使[3H]甲羟戊酸掺入蛋白质的量减少50%。在无细胞试验中，展青霉素抑制大鼠脑法尼基：蛋白质转移酶，这是一种负责蛋白质异戊二烯化的酶。在浓度为290微摩尔时抑制率为50%。
