Hsieh K H, Ng C K
Department of Pediatrics, College of Medicine, National Taiwan University, Taipei, Republic of China.
J Allergy Clin Immunol. 1993 Feb;91(2):650-7. doi: 10.1016/0091-6749(93)90271-g.
To explore the possible role of platelet-activating factor (PAF) in the pathogenesis of bronchial asthma, circulating PAF and in vitro production of PAF were studied.
Radioimmunoassay kits were used in 15 children with acute asthmatic attacks, in 25 newly diagnosed asthmatic children, in 25 good and 18 poor responders to immunotherapy, and in 18 healthy controls.
The results demonstrated the following: (1) PAF was present in the blood of healthy controls. (2) New patients had much higher circulating PAF than did healthy controls (p < 0.005), and the circulating PAF decreased after immunotherapy in good (p < 0.005) but not in poor responders. (3) The circulating PAF increased up to 20 times that of healthy controls during acute asthmatic attacks. (4) The spontaneous and allergen-stimulated secretion of PAF were markedly increased in new patients and decreased to normal after successful immunotherapy (p < 0.005). (5) No increased spontaneous and allergen-stimulated production of PAF was found during acute attacks, but granulocytes from those patients still produced the greatest amount of PAF when stimulated with calcium ionophore A23187. (6) Although a major portion of allergen-induced PAF was secreted, less than 10% of ionophore-induced PAF was secreted.
The findings that the circulating PAF increased markedly during acute asthmatic attacks and the enhanced in vivo and in vitro productions of PAF decreased to normal after successful immunotherapy strongly suggest that PAF may be involved in the pathogenesis of bronchial asthma.
为探讨血小板活化因子（PAF）在支气管哮喘发病机制中的可能作用，对循环PAF及PAF的体外生成进行了研究。
采用放射免疫分析试剂盒检测15例急性哮喘发作患儿、25例新诊断哮喘患儿、25例免疫治疗反应良好者和18例免疫治疗反应不佳者以及18例健康对照者。
结果表明：（1）健康对照者血液中存在PAF。（2）新诊断患者的循环PAF水平显著高于健康对照者（p<0.005），免疫治疗后反应良好者的循环PAF水平下降（p<0.005），而反应不佳者则未下降。（3）急性哮喘发作期间，循环PAF水平升高至健康对照者的20倍。（4）新诊断患者PAF的自发分泌和变应原刺激分泌显著增加，成功免疫治疗后降至正常水平（p<0.005）。（5）急性发作期间未发现PAF的自发分泌和变应原刺激生成增加，但这些患者的粒细胞在用钙离子载体A23187刺激时仍产生最多的PAF。（6）虽然变应原诱导的PAF大部分已分泌，但离子载体诱导的PAF分泌不到10%。
急性哮喘发作期间循环PAF显著增加，成功免疫治疗后体内和体外PAF生成增强降至正常水平，这些发现强烈提示PAF可能参与支气管哮喘的发病机制。
