Matsuo H, Maruo T, Murata K, Mochizuki M
Department of Obstetrics and Gynecology, Kobe University School of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1993 Jan;45(1):23-30.
Effects of epidermal growth factor (EGF) on the expression of trophoblast proliferation and differentiation were investigated in vitro using an organ culture system of human placental tissues. Explants of trophoblastic tissues obtained from each trimester of gestation were cultured with or without EGF (100 ng/ml) in serum-free conditions. In order to determine the possible effect of EGF on the proliferation activity of villous trophoblasts, immunostaining with monoclonal antibody Ki-67 was used. On the other hand, the possible effect of EGF on the differentiated cellular function of villous trophoblasts was assessed by determining the ability to secrete hCG and hPL. In 4-5 week placenta, EGF and EGF receptor were found to be almost exclusively localized in cytotrophoblasts and EGF enhanced the proliferation of cytotrophoblasts without affecting the ability to secrete hCG and hPL, whereas in 6-12 week placenta, EGF and EGF receptor were predominantly localized in syncytiotrophoblasts and EGF stimulated the secretion of hCG and hPL without affecting the proliferation of cytotrophoblasts. These findings suggest that EGF has dual actions which independently affect trophoblast proliferation and differentiation in the first trimester of gestation in an autocrine manner. But, in the second and third trimesters of gestation, EGF mediates the expression of placental function in a paracrine manner.
采用人胎盘组织器官培养系统，在体外研究了表皮生长因子（EGF）对滋养层细胞增殖和分化表达的影响。从妊娠各期获取的滋养层组织外植体在无血清条件下培养，添加或不添加EGF（100 ng/ml）。为了确定EGF对绒毛滋养层细胞增殖活性的可能影响，使用单克隆抗体Ki-67进行免疫染色。另一方面，通过测定分泌hCG和hPL的能力来评估EGF对绒毛滋养层细胞分化细胞功能的可能影响。在4 - 5周的胎盘中，发现EGF和EGF受体几乎仅定位于细胞滋养层，EGF增强了细胞滋养层的增殖，而不影响分泌hCG和hPL的能力；而在6 - 12周的胎盘中，EGF和EGF受体主要定位于合体滋养层，EGF刺激了hCG和hPL的分泌，而不影响细胞滋养层的增殖。这些发现表明，EGF具有双重作用，在妊娠早期以自分泌方式独立影响滋养层细胞的增殖和分化。但是，在妊娠中期和晚期，EGF以旁分泌方式介导胎盘功能的表达。
