Hahn S L, Hahn M, Kang U J, Joh T H
Laboratory of Molecular Neurobiology, Burke Medical Research Institute, Cornell University Medical College, White Plains, New York 10605.
J Neurochem. 1993 Mar;60(3):1058-64. doi: 10.1111/j.1471-4159.1993.tb03254.x.
Aromatic L-amino acid decarboxylase catalyzes the biosynthesis of the neurotransmitters dopamine and serotonin. This enzyme is also expressed in nonneuronal tissues. Two reported cDNA sequences show that the pheochromocytoma message differs from the liver message only at the 5' untranslated region. We present the complete exonal organization and promoter sequences of the rat gene encoding this enzyme. The rat aromatic L-amino acid decarboxylase gene is composed of two promoters and 16 exons spanning more than 80 kb in the genome. The first exon carries the majority of the 5' untranslated sequence of the liver cDNA, and the second exon carries that of the pheochromocytoma cDNA. In the third exon, there are two alternatively utilized splicing acceptors specific to the first and second exons. Therefore, both alternative promoter usage and alternative splicing are operative for the differential expression of this gene. The sequence of each promoter region shows putative binding sites for octamer factors and AP-2.
芳香族L-氨基酸脱羧酶催化神经递质多巴胺和5-羟色胺的生物合成。该酶也在非神经组织中表达。两个已报道的cDNA序列显示，嗜铬细胞瘤的信息与肝脏的信息仅在5'非翻译区有所不同。我们展示了编码该酶的大鼠基因的完整外显子组织和启动子序列。大鼠芳香族L-氨基酸脱羧酶基因由两个启动子和16个外显子组成，在基因组中跨越超过80kb。第一个外显子携带肝脏cDNA大部分的5'非翻译序列，第二个外显子携带嗜铬细胞瘤cDNA的该序列。在第三个外显子中，有两个分别特异于第一和第二个外显子的可变剪接受体。因此，启动子的交替使用和可变剪接都对该基因的差异表达起作用。每个启动子区域的序列显示了八聚体因子和AP-2的假定结合位点。
