Hilf M E, Dawson W O
Department of Plant Pathology, University of California, Riverside 92521.
Virology. 1993 Mar;193(1):106-14. doi: 10.1006/viro.1993.1107.
The tobamoviruses, tobacco mosaic virus (TMV) and Odontoglossum ringspot virus (ORSV), differ in the range of plant species that each can systemically infect. Both viruses systemically infect Nicotiana benthamiana similarly, but differ in the ability to systemically infect tobacco (N. tabacum). ORSV is confined to the inoculated leaves of N. tabacum, whereas TMV causes a rapid systemic infection. Genetic chimeras were created to identify viral genes involved in specific interactions in long-distance movement in N. tabacum. In N. tabacum, a chimera expressing the ORSV 30-kDa protein exhibited a reduced rate of cell-to-cell spread, similar to that of ORSV, and, also like ORSV, was deficient in systemic invasion, implying a relationship between the rates of cell-to-cell and long-distance movement. However, a TMV chimera expressing the ORSV capsid protein gene spread cell to cell similarly to TMV, but was deficient in long-distance movement and systemic infection. These data suggest that tobamovirus capsid protein(s) may be required to interact with host components in a specific manner to allow efficient long-distance movement, and the ORSV capsid protein did not function in this manner in tobacco plants.
烟草花叶病毒属病毒，烟草花叶病毒（TMV）和齿舌兰环斑病毒（ORSV），在各自能够系统感染的植物物种范围上存在差异。两种病毒对本氏烟草的系统感染情况相似，但在对烟草（烟草）的系统感染能力上有所不同。ORSV局限于烟草的接种叶片，而TMV会引发快速的系统感染。构建了基因嵌合体以鉴定参与烟草长距离移动中特定相互作用的病毒基因。在烟草中，表达ORSV 30 kDa蛋白的嵌合体表现出细胞间传播速率降低，与ORSV相似，并且，也与ORSV一样，在系统侵染方面存在缺陷，这意味着细胞间和长距离移动速率之间存在关联。然而，表达ORSV衣壳蛋白基因的TMV嵌合体在细胞间的传播方式与TMV相似，但在长距离移动和系统感染方面存在缺陷。这些数据表明，烟草花叶病毒属病毒的衣壳蛋白可能需要以特定方式与宿主成分相互作用，以实现高效的长距离移动，而ORSV衣壳蛋白在烟草植株中并非以这种方式发挥作用。
