Ni Y, Ramig R F, Kemp M C
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467.
Virology. 1993 Mar;193(1):466-9. doi: 10.1006/viro.1993.1147.
Twelve avian reovirus strain 176 proteins, 10 structural (lambda 1, lambda 2, lambda 3, mu 1, mu 2/mu 2C, sigma 1, sigma 2, sigma 3, and sigma 4), and 2 nonstructural (mu NS and sigma NS) were identified and characterized by electrophoretic analysis of purified virions and infected cell lysates. Three of the identified proteins (mu 1, mu NS, and sigma 4) have not been previously described. In pulse-chase experiments, 10 of the proteins (lambda 1, lambda 2, lambda 3, mu 1, mu 2, mu NS, sigma 1, sigma 2, sigma NS, sigma 3) were shown to be primary translation products, whereas mu 2C was shown to be a post-translational cleavage product of mu 2.
通过对纯化病毒粒子和感染细胞裂解物进行电泳分析，鉴定并表征了12种禽呼肠孤病毒176株蛋白，其中10种为结构蛋白（λ1、λ2、λ3、μ1、μ2/μ2C、σ1、σ2、σ3和σ4），2种为非结构蛋白（μNS和σNS）。已鉴定出的3种蛋白（μ1、μNS和σ4）此前尚未见报道。在脉冲追踪实验中，10种蛋白（λ1、λ2、λ3、μ1、μ2、μNS、σ1、σ2、σNS、σ3）被证明是初级翻译产物，而μ2C被证明是μ2的翻译后切割产物。
