Mutani R, Durelli L, Mazzarino M, Valentini C, Monaco F, Fumero S, Mondino A
Brain Res. 1977 Feb 25;122(3):513-21. doi: 10.1016/0006-8993(77)90461-9.
In cats affected with cortical epileptogenic foci induced by penicillin application to and cobalt implantation into the pericruciate area, the brain amino acids contents were determined in the focus as well as in extrafocal areas. In different groups of animals, brain removal for biochemical determinations was performed at different times before, during and after epilepsy and the values compared to controls. The only amino acid to show a significant change before appearance of spikes in both types of epilepsy was taurine, which decreased. Cobalt epilepsy was accompanied by changes in a larger number of amino acids than penicillin epilepsy: in the former the brain content of taurine, GABA, aspartate, glutamate, serine, threonine, glycine and alanine was altered. The changes were proportional to the severity of epilepsy and more prominent in the focus area. After disappearance of spikes the levels of most amino acids returned to normal except for some amino acids, previously unaffected by penicillin epilepsy, which were decreased. It is proposed that the decrease in brain taurine, occurring before the appearance of penicillin and cobalt epilepsy, could increase the excitability of a certain neuronal population and thus, by potentiating the effects on neurons of penicillin and cobalt, contribute to the initiation of epilepsy.
在通过向十字沟周围区域应用青霉素和植入钴诱导产生皮质致痫灶的猫中，测定了病灶以及病灶外区域的脑氨基酸含量。在不同组的动物中，在癫痫发作前、发作期间和发作后的不同时间进行脑部切除以进行生化测定，并将这些值与对照组进行比较。在两种类型的癫痫发作出现棘波之前，唯一显示出显著变化的氨基酸是牛磺酸，其含量降低。与青霉素癫痫相比，钴癫痫伴有更多氨基酸的变化：在前者中，牛磺酸、γ-氨基丁酸、天冬氨酸、谷氨酸、丝氨酸、苏氨酸、甘氨酸和丙氨酸的脑含量发生了改变。这些变化与癫痫的严重程度成正比，且在病灶区域更为明显。在棘波消失后，除了一些之前不受青霉素癫痫影响但含量降低的氨基酸外，大多数氨基酸水平恢复正常。有人提出，在青霉素和钴癫痫发作出现之前脑牛磺酸含量的降低可能会增加某些神经元群体的兴奋性，从而通过增强青霉素和钴对神经元的作用，促成癫痫的发作。
