Seiki M
Dept. of Virology, Cancer Research Institute, Kanazawa University.
Gan To Kagaku Ryoho. 1993 Feb;20(3):387-92.
Tumor cells have to degrade extracellular matrix components to invade surrounding tissue and to form metastatic colonies at distant organs. Matrix metalloproteinases are the enzymes that can degrade various types of native collagens and glycoproteins. In this study, we demonstrated the roles of matrix metalloproteinases in metastatic colony formation in chick embryo by transfecting TIMP-1 gene into a metastatic gastric cancer cell line, KKLS. The mechanism regulating expression of one of the type IV collagenases, MMP-9, was also studied using the promoter region of the gene.
肿瘤细胞必须降解细胞外基质成分，以侵入周围组织并在远处器官形成转移瘤。基质金属蛋白酶是能够降解各种类型天然胶原蛋白和糖蛋白的酶。在本研究中，我们通过将TIMP-1基因转染至转移性胃癌细胞系KKLS中，证明了基质金属蛋白酶在鸡胚转移瘤形成中的作用。我们还利用该基因的启动子区域研究了IV型胶原酶之一MMP-9的表达调控机制。
