Gross P M, Weaver D F, Wainman D S, Chew B H, Espinosa F J, Nag S
Department of Surgery, Queen's University and Kingston General Hospital, Ontario, Canada.
Biochem Biophys Res Commun. 1993 Feb 15;190(3):975-81. doi: 10.1006/bbrc.1993.1145.
Endothelin-1 (ET) and sodium nitroprusside (SNP, which liberates nitric oxide, NO) were given alone or together into a lateral cerebral ventricle (icv) of anesthetized rats to assess their potential interaction on cerebral rates of glucose metabolism (autoradiographic [14C]deoxyglucose technique). ET (9 pmol) produced hypermetabolic effects ipsilaterally in the septal nuclei and periventricular white matter. NO lesioned the septum, which displayed neuronal damage and diminished metabolic activity, and evoked potent increases in glucose metabolism bilaterally in commissural and projection white matter tracts. Together, ET and NO had synergistic hypermetabolic effects in the hippocampal fimbria, but were antagonistic on the metabolic rate of the lateral septal nucleus and choroid plexus. The results reveal an extraordinary sensitivity in the metabolic rate of septal gray matter to ET and of white matter fibers to NO in vivo. Icv administration offers a useful approach for examination of the metabolic and toxicological properties of the novel neurotransmitter substances ET and NO on septal neurons, myelinated fibers, and choroidal epithelia.
将内皮素-1（ET）和硝普钠（SNP，可释放一氧化氮，NO）单独或联合注入麻醉大鼠的侧脑室（icv），以评估它们对脑葡萄糖代谢率的潜在相互作用（放射自显影[14C]脱氧葡萄糖技术）。ET（9皮摩尔）在同侧的隔核和室周白质产生代谢亢进效应。NO损害隔区，表现为神经元损伤和代谢活性降低，并在双侧连合和投射白质束中引起葡萄糖代谢显著增加。ET和NO共同作用时，在海马伞中有协同的代谢亢进效应，但对外侧隔核和脉络丛的代谢率起拮抗作用。结果表明，在体内隔区灰质的代谢率对ET以及白质纤维的代谢率对NO具有极高的敏感性。侧脑室给药为研究新型神经递质ET和NO对隔区神经元、有髓纤维和脉络丛上皮的代谢及毒理学特性提供了一种有用的方法。
