Muguruma K, Komatz Y, Ikeda M, Sugimoto T, Saito K
Department of Urology, Kansai Medical University, Osaka, Japan.
Biol Reprod. 1993 Feb;48(2):386-92. doi: 10.1095/biolreprod48.2.386.
Studies were conducted on platelet-activating factor (PAF) in male reproductive organs. Total lipids were extracted from the seminal vesicles, and PAF was purified by silicic column chromatography, thin-layer chromatography, and HPLC. PAF activities in the seminal vesicles of guinea pigs and rats as measured by assay of platelet aggregation were 0.26 and 0.25 ng PAF/mumol original phospholipids. These activities were inhibited by the specific PAF antagonists CV6209 and L652,731. In guinea pigs, PAF activity decreased to 50% and 31% of the normal level 2 wk and 4 wk, respectively, after castration. The activity increased to 136% of the normal level on administration of 1 mg of testosterone propionate every day for 1 wk beginning 2 wk after castration, but decreased to 74% of the control level on administration of vehicle. Furthermore, administration of 100 IU of hCG every other day to guinea pigs resulted in increased activity, to 132% (p < 0.05) and 154% (p < 0.01) of the activity in vehicle-treated animals on Days 1 and 3, respectively. Electron microscopic studies showed changes in the rough endoplasmic reticulum of the epithelial cells of the seminal vesicles in castrated guinea pigs. (The rough endoplasmic reticulum is involved in PAF biosynthesis.) PAF activity was also demonstrated in tissues of the testis, epididymis, and vas deferens of guinea pigs and rats, and in the prostate of rats. Thus PAF may play important physiological roles in male reproductive organs.
对雄性生殖器官中的血小板活化因子（PAF）进行了研究。从精囊中提取总脂质，通过硅胶柱色谱、薄层色谱和高效液相色谱法纯化PAF。通过血小板聚集测定法测得豚鼠和大鼠精囊中PAF的活性分别为0.26和0.25 ng PAF/μmol原始磷脂。这些活性受到特异性PAF拮抗剂CV6209和L652,731的抑制。在豚鼠中，去势后2周和4周，PAF活性分别降至正常水平的50%和31%。去势后2周开始每天给予1 mg丙酸睾酮，持续1周，活性增加至正常水平的136%，但给予赋形剂后降至对照水平的74%。此外，每隔一天给豚鼠注射100 IU人绒毛膜促性腺激素（hCG），在第1天和第3天，活性分别增加至给予赋形剂处理动物活性的132%（p < 0.05）和154%（p < 0.01）。电子显微镜研究显示，去势豚鼠精囊上皮细胞的粗面内质网发生了变化。（粗面内质网参与PAF的生物合成。）在豚鼠和大鼠的睾丸、附睾和输精管组织以及大鼠的前列腺中也证实了PAF活性。因此，PAF可能在雄性生殖器官中发挥重要的生理作用。
