Fulthorpe R R, Liss S N, Allen D G
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Ont., Canada.
Can J Microbiol. 1993 Jan;39(1):13-24. doi: 10.1139/m93-003.
Water samples from the wastewater treatment system of a bleach kraft mill and from the river that supplies the mill were plated on six different media and culturable isolates were screened for substrate utilization patterns, taxonomic characters, plasmid content, and resistance to ampicillin, streptomycin, kanamycin, tetracycline, naldixic acid, mercury, nickel, copper, cobalt, cadmium, and zinc. A cluster analysis of the substrate utilization profiles and taxonomic characters revealed that Pseudomonas, Acinetobacter, and Acidovorax spp. were common among the culturable isolates from the river, while Ancylobacter aquaticus, Klebsiella spp., and an unknown group of pleomorphic Gram-negative methylotrophs were common among the culturable isolates from the mill treatment system. Of isolates from the settling pond and aerated lagoon, 78 and 64% carried plasmids, while only 56% of isolates from the river carried plasmids. Plasmids were significantly associated with resistance to cadmium but not with any other resistance characters. Large numbers of plasmid-carrying A. aquaticus strains and pleomorphic methylotrophs accounted for high plasmid incidence levels in the mill treatment system, and the ability to dechlorinate simple aliphatic substrates was found in these two groups as well as in one Pseudomonas strain.
从一家漂白硫酸盐浆厂的废水处理系统以及为该厂供水的河流中采集水样，将其接种于六种不同培养基上，并对可培养分离株进行筛选，以检测其底物利用模式、分类学特征、质粒含量以及对氨苄青霉素、链霉素、卡那霉素、四环素、萘啶酸、汞、镍、铜、钴、镉和锌的抗性。对底物利用谱和分类学特征进行聚类分析后发现，假单胞菌属、不动杆菌属和嗜酸菌属在河流的可培养分离株中较为常见，而水生柄杆菌、克雷伯菌属以及一组未知的多形革兰氏阴性甲基营养菌在工厂处理系统的可培养分离株中较为常见。在沉淀池和曝气塘的分离株中，分别有78%和64%携带质粒，而河流中的分离株只有56%携带质粒。质粒与对镉的抗性显著相关，但与其他抗性特征无关。大量携带质粒的水生柄杆菌菌株和多形甲基营养菌导致了工厂处理系统中较高的质粒发生率，并且在这两组以及一株假单胞菌菌株中发现了对简单脂肪族底物进行脱氯的能力。
