Fulthorpe R R, Allen D G
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Canada.
Appl Microbiol Biotechnol. 1995 Jan;42(5):782-9. doi: 10.1007/BF00171962.
The relative importance of each of three dechlorinating species to overall organochlorine removal from bleached kraft-mill effluents (BKME) was assessed. Ancylobacter aquaticus A7, Pseudomonas P1, and Methylobacterium CP13, strains indigenous to a BKME treatment system, were tested for growth on chlorinated acetic acids and alcohols, and for adsorbable organic halogen (AOX) reduction in batch cultures of sterile BKME from three sources. A. aquaticus A7 exhibited the broadest substrate range, but could only affect significant AOX reduction in softwood effluents. Methylobacterium CP13 exhibited a limited substrate range, but was capable of removing significant amounts of AOX from both hardwood and softwood effluents. By contrast, Pseudomonas sp. P1 exhibited a limited substrate range and poor to negligible reductions in AOX levels from both effluent types. Mixed inocula of all three species combined and inocula of sludge from mill treatment systems removed as much AOX from softwood effluents as did pure populations of Methylobacterium CP13. When BKME was hydrolysed prior to AOX analysis, the subsequent estimates of recalcitrant, or non-hydrolysable, AOX levels were far less variable than their counterpart total AOX measures. It is suggested that this is a relevant and useful measure of AOX for biodegradation studies.
评估了三种脱氯菌对从漂白硫酸盐制浆厂废水（BKME）中去除总有机氯的相对重要性。测试了BKME处理系统中的本地菌株水生柄杆菌A7、假单胞菌P1和甲基杆菌CP13在氯化乙酸和醇类上的生长情况，以及在来自三个来源的无菌BKME分批培养物中对可吸附有机卤素（AOX）的还原能力。水生柄杆菌A7表现出最广泛的底物范围，但仅能在针叶木废水中显著降低AOX。甲基杆菌CP13的底物范围有限，但能够从阔叶木和针叶木废水中去除大量AOX。相比之下，假单胞菌P1的底物范围有限，两种废水类型中AOX水平的降低效果较差或可忽略不计。这三种菌株的混合接种物以及制浆厂处理系统的污泥接种物从针叶木废水中去除的AOX与甲基杆菌CP13的纯培养物相当。在对AOX进行分析之前对BKME进行水解时，随后对难降解或不可水解的AOX水平的估计值比相应的总AOX测量值的变异性小得多。建议这是生物降解研究中一种相关且有用的AOX测量方法。
