Vardi E, Grover N B
Hubert H. Humphrey Center for Experimental Medicine and Cancer Research, Hebrew University Faculty of Medicine, Jerusalem, Israel.
Cytometry. 1993;14(2):173-8. doi: 10.1002/cyto.990140209.
We have investigated the phenomenon of shape distortion in a sample of 1,552 Escherichia coli B/r A cells in balanced exponential growth, during preparation for electron microscopy by agar filtration. Mixed preparations of bacterial cells and polystyrene latex spheres were shadow cast at low angle and the resulting shadows used to obtain quantitative estimates for the dimensions of the dehydrated cells; these then serve as a basis for a model of its shape in three dimensions. A statistical analysis of the projections of clustered cells and the intervening fissures, in nonshadow-cast preparations, provides an estimate of the effects of drying. The average width of the dehydrated cell (450 nm) is about 20 nm greater than the diameter of the live bacterium, whereas its length (1,398 nm) is approximately 40 nm less.
我们研究了在通过琼脂过滤法制备用于电子显微镜观察的样本中，处于平衡指数生长期的1552个大肠杆菌B/r A细胞的形状畸变现象。将细菌细胞和聚苯乙烯乳胶球的混合制剂以低角度进行投影，然后利用所得投影对脱水细胞的尺寸进行定量估计；这些估计值随后作为其三维形状模型的基础。对未进行投影的聚簇细胞和其间裂缝的投影进行统计分析，可估计干燥的影响。脱水细胞的平均宽度（450纳米）比活细菌的直径大约20纳米，而其长度（1398纳米）则大约短40纳米。
