Kubohara Y, Okamoto K
Department of Botany, Faculty of Science, Kyoto University, Japan.
Exp Cell Res. 1993 Feb;204(2):382-4. doi: 10.1006/excr.1993.1047.
In submerged culture, the prespore-specific gene, D19, of Dictyostelium discoideum was found to be expressed in the early stages of development, even in the presence of 1 nM of DIF-1 (stalk differentiation inducing factor). This concentration of DIF-1 later caused the degradation of the previously accumulated D19 mRNA concomitant with the induction of the prestalk-specific genes ecmA/ecmB and eventually 85% of cells differentiated into stalk cells. These results suggest that prespore differentiation occurs at least transiently even in the presence of DIF-1.
在深层培养中，发现盘基网柄菌的前孢子特异性基因D19在发育早期表达，即使存在1 nM的DIF-1（柄细胞分化诱导因子）。这种浓度的DIF-1随后导致先前积累的D19 mRNA降解，同时诱导前柄特异性基因ecmA/ecmB，最终85%的细胞分化为柄细胞。这些结果表明，即使存在DIF-1，前孢子分化至少会短暂发生。
